modified GRF(1-29) raises a handful of sensible questions. This page answers them in order, starting with the fundamentals and moving to applications.
Reviewed 2026-05-11. Anything still debated is marked as such rather than presented as settled.
At the pituitary, the peptide binds the growth hormone-releasing hormone receptor on somatotroph cells. Receptor activation raises intracellular cyclic AMP and triggers release of stored growth hormone. Somatostatin and other hypothalamic signals modulate this response. Negative feedback from insulin-like growth factor 1 also influences output. The same regulatory architecture operates with the native hormone. Whether the synthetic analog alters feedback dynamics over repeated exposure remains an open question. Most published receptor work uses cell models rather than intact human systems.
CJC-1295 is a synthetic peptide belonging to the growth hormone-releasing hormone analog family. It comprises twenty-nine amino acid residues derived from the N-terminal region of natural GHRH. The molecule incorporates several non-natural substitutions that increase resistance to enzymatic degradation. These modifications extend its activity compared with the native hormone fragment. Researchers use it to study pituitary growth hormone secretion in laboratory and clinical settings. This compound is distinct from native GHRH in its stability profile.
Two forms circulate in research settings and are frequently confused. One carries the drug affinity complex and is often written as CJC-1295 with DAC; the other lacks that group and is usually called modified GRF(1-29). The two share the same core sequence but differ sharply in how long they persist in blood. Products labelled only as CJC-1295 normally refer to the version carrying the complex. Documentation that omits the distinction leaves the intended molecule ambiguous.
CJC-1295 is a synthetic peptide built as a long-acting analogue of growth hormone-releasing hormone. Its backbone matches the first twenty-nine residues of the natural human hormone, with four amino acid substitutions added to slow enzymatic breakdown. A reactive maleimide group, commonly termed the drug affinity complex, allows the peptide to attach to circulating albumin after administration. That albumin attachment keeps the molecule in the bloodstream for an extended period instead of being cleared within minutes.
The compound emerged from work at a Canadian biotechnology firm in the early 2000s. Early human studies examined its effect on growth hormone and insulin-like growth factor 1 in healthy volunteers and in people with HIV-associated fat redistribution. Reports described sustained increases in both markers after a single injection. Development did not advance to regulatory approval, and the clinical programme was later discontinued. The molecule is now encountered mainly as a research chemical rather than a marketed medicine.
| Property | Value | Notes |
|---|---|---|
| Molecular weight, DAC form | About 3647 Da | Varies with salt form |
| Molecular weight, non-DAC form | About 3358 Da | MOD GRF(1-29) |
| Solubility class | Water soluble | Also dissolves in polar solvents |
| Reported half-life, DAC form | About five to eight days | Values from human studies |
| Common synonyms | CJC-1295; MOD GRF(1-29) | Name depends on variant |
The albumin-binding version stays in circulation for days, because covalent attachment to serum albumin shields the peptide from rapid filtration and degradation. Reported half-lives for this form fall in the range of several days. The version without the linker is cleared in minutes, with estimates often near thirty minutes in animal work. These figures come from small studies and vary with assay method, species, and route, so they are best read as approximate rather than fixed constants.
Studies in this area generally track growth hormone pulses, insulin-like growth factor 1 concentrations, and occasionally body composition endpoints. Most published human data come from early, small trials, and questions about long-term effects remain open. Whether repeated exposure alters pituitary responsiveness over time is not settled. Analytical work relies on immunoassays for the hormones and on mass spectrometry for the peptide itself, because the two measurements answer different questions.
Binding of the peptide to the growth hormone-releasing hormone receptor on pituitary somatotrophs triggers a G protein coupled cascade that raises cyclic AMP and opens calcium channels. The result is greater secretion of growth hormone into the bloodstream. Because the peptide acts at the same receptor as the natural hypothalamic hormone, its effect is amplified pulse size rather than an entirely separate release pathway. Receptor binding alone does not determine the response, since somatostatin tone and other inputs modulate the final output.
Purity is most often assessed by reversed-phase high-performance liquid chromatography, reported as a percentage of total peak area. Identity is confirmed by mass spectrometry, which yields a molecular ion consistent with the expected sequence. Amino acid analysis and peptide mapping provide additional characterization. Reported purity values are method-dependent, so figures from different laboratories are not always directly comparable without details of column, gradient, and detection wavelength.
Peptide degradation proceeds mainly through hydrolysis, oxidation of methionine, and deamidation of asparagine or glutamine residues. The maleimide group on the albumin-binding variant can also react with thiols or hydrolyze in aqueous media. Because these pathways accelerate with temperature and pH extremes, handling conditions strongly influence measured stability. Stability data in the public literature are limited and often generated under differing conditions, so general statements about shelf life should be read as approximate.
=== Further manifestations of solvent hydrogen bonding === Increase in the melting point, boiling point, solubility, and viscosity of many compounds can be explained by the concept of hydrogen bonding. Negative azeotropy of mixtures of HF and water. The fact that ice is less dense than liquid water is due to a crystal structure stabilized by hydrogen bonds. Dramatically higher boiling points of NH3, H2O, and HF compared to the heavier analogues PH3, H2S, and HCl, where hydrogen-bonding is absent. Viscosity of anhydrous phosphoric acid and of glycerol. Dimer formation in carboxylic acids and hexamer formation in hydrogen fluoride, which occur even in the gas phase, resulting in gross deviations from the ideal gas law. Pentamer formation of water and alcohols in apolar solvents.
Supermarket News ranked 7-Eleven's North American operations No. 11 in the 2007 "Top 75 North American Food Retailers", based on the 2006 fiscal year estimated sales of US$15.0 billion. Based on the 2005 revenue, 7-Eleven is the 24th largest retailer in the United States. As of 2013, 8,144 7-Eleven franchised units exist across the United States. Franchise fees range between US$10,000 – $1,000,000 and the ongoing royalty rate varies. 7-Eleven America has its headquarters in the Cypress Waters development in Irving, Texas. Small-size Slurpees are free on "7-Eleven Day", on July 11. This holiday first became widely celebrated on July 11, 2008, when first discovered by J. Brabank and C. Johnson. One exception is 2020, when the COVID-19 pandemic caused that year's cancellation. 7 Rewards members got a free medium Slurpee in their app instead. 7-Eleven Stores of Oklahoma operated independently beginning in 1953 under an agreement with the Brown family. As part of this franchise agreement, 7-Elevens in Oklahoma bore slight differences to stores elsewhere: for instance, products such as Big Bite hot dogs were not sold there, the Slurpee was branded as the "Icy Drink", and Oklahoma stores operated their own loyalty program called "Thx!", which did not intersect with the national 7Rewards system. On March 2, 2020, 7-Eleven, Inc. announced it had officially closed on the acquisition of over 100 of these independently operated 7-Eleven stores in Oklahoma. All of these 100 stores were in the greater Oklahoma City metropolitan area.
Flay opened a second Mesa Grill at Caesars Palace in Las Vegas in 2004, and in 2005 he opened Bar Americain, an American Brasserie, in Midtown Manhattan. He continued to expand his restaurants by opening Bobby Flay Steak in the Borgata Hotel Casino & Spa in Atlantic City, New Jersey. This was followed by a third Mesa Grill in the Bahamas, located in The Cove at Atlantis Paradise Island, which opened on March 28, 2007. The Las Vegas Mesa Grill earned Flay his only Michelin Star in 2008, which was taken away in the 2009 edition. Michelin did not publish a 2010 or 2011 Las Vegas edition, so the star could not be re-earned. Bolo Bar & Restaurant closed its doors on December 31, 2007, to make way for a condominium. Aside from his restaurants and television shows, Flay has been a master instructor and visiting chef at the French Culinary Institute. Although he is not currently teaching classes, he occasionally visits when his schedule permits. Flay established the Bobby Flay Scholarship in 2003. This full scholarship to the French Culinary Institute is awarded annually to a student in the Long Island City Culinary Arts Program. Flay personally helps select the awardee each year. Flay opened his first Bobby's Burger Palace (BBP) at the Smith Haven Mall in Lake Grove, Long Island, on July 15, 2008. At its peak, BBP had nineteen locations in eleven states and the District of Columbia. The original Mesa Grill in New York closed in September 2013 following a proposed rent increase by the landlord.
== Additional references == Bodenhamer, David J; Barrows, Robert G, eds. (1994). The Encyclopedia of Indianapolis. Bloomington and Indianapolis: Indiana University Press. ISBN 978-0-253-31222-8. Kahn, E. J. (1975). All In A Century: The First 100 Years of Eli Lilly and Company. West Cornwall, CT: Eli Lilly and Company. OCLC 5288809. Podczeck, Fridrun; Jones, Brian E. (2004). Pharmaceutical Capsules. Chicago: Pharmaceutical Press. ISBN 978-0-85369-568-4. Price, Nelson (1997). Indiana Legends: Famous Hoosiers From Johnny Appleseed to David Letterman. Indianapolis: Guild Press of Indiana. ISBN 978-1-57860-006-9. Taylor Jr., Robert M.; Stevens, Errol Wayne; Ponder, Mary Ann; Brockman, Paul (1989). Indiana: A New Historical Guide. Indianapolis: Indiana Historical Society. p. 481. ISBN 978-0-87195-048-2. Tobias, Randall; Tobias, Todd (2003). Put the Moose on the Table: Lessons in Leadership from a CEO's Journey through Business and Life. Indiana University Press. ISBN 978-0-253-11011-4. Weintraut, Linda; Nolan, Jane R. "The Secret Life of Building 314". Traces of Indiana and Midwestern History. 8 (3). Indianapolis: Indiana Historical Society: 16–27.
== Trials == Howard Florey's team at Oxford showed that Penicillium extract killed many kinds of bacteria. Gardner and Orr-Ewing tested it against gonococcus (against which it was most effective), meningococcus, streptococcus, staphylococcus, Bacillus anthracis, actinomyces and tetanus bacterium (Clostridium tetani) and the bacteria that cause gangrene. They observed bacteria attempting to grow in the presence of penicillin, and noted that penicillin was neither an enzyme that broke the bacteria down, nor an antiseptic that killed them; rather, it was a chemical that interfered with the process of cell division. Jennings observed that it had no effect on white blood cells, and would therefore reinforce rather than hinder the body's natural defences against bacteria. She also found that unlike sulphonamides, the first and only effective broad-spectrum antibiotic available at the time, it was not destroyed by pus. Medawar found that it did not affect the growth of tissue cells.
Sources: en.wikipedia.org
=== 2010 census === As of the census of 2010, there were 4,025 people, 1,549 households, and 1,049 families living in the city. The population density was 1,507.5 inhabitants per square mile (582.0/km2). There were 1,641 housing units at an average density of 614.6 units per square mile (237.3 units/km2). The racial makeup of the city was 94.2% White, 0.2% African American, 0.2% Native American, 0.2% Asian, 0.1% Pacific Islander, 3.9% from other races, and 1.0% from two or more races. Hispanic or Latino of any race were 7.1% of the population. There were 1,549 households, of which 36.6% had children under the age of 18 living with them, 53.6% were married couples living together, 10.1% had a female householder with no husband present, 4.0% had a male householder with no wife present, and 32.3% were non-families. 27.8% of all households were made up of individuals, and 16.4% had someone living alone who was 65 years of age or older. The average household size was 2.54 and the average family size was 3.11. The median age in the city was 36.7 years. 27.7% of residents were under the age of 18; 6.5% were between the ages of 18 and 24; 26.4% were from 25 to 44; 20.4% were from 45 to 64; and 19% were 65 years of age or older. The gender makeup of the city was 48.3% male and 51.7% female. Ancestry breakdown of residents is German (67.6%), American (5.9%), Swedish (4.0%), Irish (2.4%), Norwegian (1.7%), English (1.2%).
== Mode of action classification == IRAC publishes an insecticide mode of action (MoA) classification that lists most common insecticides and acaricides and recommends that "successive generations of a pest should not be treated with compounds from the same MoA Group". IRAC assigns a mode of action (MoA) to an insecticide, based on sufficient scientific data. They then update the mode of action (MoA) classification. Several insecticides and classes of insecticide may act through the same mode of action.
=== Diffusion across the surface === The high surface area of a material in nanoparticle form allows heat, molecules, and ions to diffuse into or out of the particles at very large rates. The small particle diameter, on the other hand, allows the whole material to reach homogeneous equilibrium with respect to diffusion in a very short time. Thus many processes that depend on diffusion, such as sintering can take place at lower temperatures and over shorter time scales which can be important in catalysis.
The electrophoretic linear (horizontal) separation of proteins by Ip along a pH gradient in a polyacrylamide gel (also known as isoelectric focusing), followed by a standard molecular weight linear (vertical) separation in a second polyacrylamide gel (SDS-PAGE), constitutes the so called two-dimensional gel electrophoresis or PAGE 2D. This technique allows a thorough separation of proteins as distinct "spots", with proteins of high molecular weight and low Ip migrating to the upper-left part of the bidimensional gel, while proteins with low molecular weight and high Ip locate to the bottom-right region of the same gel.
Sources: en.wikipedia.org
It is a synthetic peptide modeled on growth hormone-releasing hormone. The molecule is used in research on pituitary growth hormone secretion. It differs from the natural hormone through several stabilizing substitutions.
The DAC form carries a maleimide group that binds albumin and extends circulation time. The non-DAC form lacks this group and clears much faster. Both share the same receptor-binding core.
It targets the growth hormone-releasing hormone receptor on pituitary somatotroph cells. Activation raises cyclic AMP and promotes growth hormone release. Normal feedback pathways remain part of the response.
It is a synthetic peptide analogue of growth hormone-releasing hormone. Four substitutions in its sequence make it more resistant to enzymatic degradation than the natural hormone. In the version carrying a drug affinity complex, the peptide binds albumin and remains in circulation for days.